How do we calculate the %hemolysis of postive and negative control in rapid invitro hemolytic assay?

Recently I read many papers of reputed journals demonstrating the invitro cytotoxcity of synthetic materials, plant extracts, particles by Hemolytic assay, Triton-X100 is generally used as...

31 December 2018 4,725 7 View

How to contact a Prof. if your govt is willing to fund your PhD ?

My Provincial Govt has started a program, that if a student have offer letter from QS top 50 universities subject wise , they will bear all expenses from boarding to academic fee. I want to...

02 March 2018 8,391 2 View

How to separate salt and hyrolyzed product while doing acidic / basic hydrolysis of gum/mucilage ?

Im try the hydrolysis following method below . The question is that we use acid afterwards for neutralization of product the salt formation takes place it also settles down along with product,...

01 February 2018 9,602 4 View

Please refer me any reproducible Green synthesis method for ZnO nanoparticles ?

I'm searching a reproducible method for green synthesis of ZnO nanoparticles by using plant extract as reducing agent which yield solid particles in quantity 1gm,

01 February 2018 2,279 5 View

What is scope scope and future opportunities after doning Masters in Education? especially if its done along with PhD in some science subject?

I need to ask two things 1. Whats the scope of masters in Education? i.e. future outcomes, job etc. 2. Is there benefit of master is education if a candidate is having along with PhD in some...

11 December 2017 4,349 4 View

What are some other certifcations, courses that are useful while pursing Phd in chemistry?

I want to ask about courses, certifications or something like that is useful along with PhD in chemistry for personal development and knowledge building as well as those which are helpful in...

11 December 2017 5,123 4 View

Can we publish antioxidant, antimicrobial, hemolysis, carcinogenicity assay, DNA damaging without chracterization techniques in a good journal?

Actually i wanted to asked if I carryout detailed Biochemical profiling study by means of TPC,TFC, DPPH,TOC, redcuing power, antimicrobial, mic, ames, hemolytic , dna damanging study on natural...

11 December 2017 114 6 View

Can anyone help how to make comaprsion graphs of XRD any software?

08 September 2017 6,354 9 View

Can anyone tell me that the base peaks at 55 and 95 what will be the structure of fargment and what do they represent?

the figure show the gc-ms of essentail oil extracted from the seeds and leaves of plant. Guide me how to explain this especially base peak 55 and 97 what do they represent ?ot tell me how to...

07 August 2017 2,073 8 View

I need different synthesis or production methods for potassium citrate help me?

11 December 2016 5,083 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View