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How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Is there any formula which is used to calculate the value of capacitive contribution in the case of battery type electrode?

Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...

12 May 2024 3,261 0 View

Does multivariate NeuralProphet need future regressor to predict the future ?

Hello everyone,I am currently working with the multivariate time series forecasting by using NeuralProphet to predict the price of product.I used 7 economic indicators as regressor in my...

22 March 2024 8,265 3 View

I would like to know that prophet time series forecasting is under the category of machine learning or neural network?

I would like to know that prophet time series model is under the category of neural network or machine learning or deep learning? I want to forecast the price of product depending on other...

19 February 2024 1,243 1 View

How to do Strain controlled cyclic triaxial testing using GDS ELDYN triaxial set up?

Dear RG users, I hope all of you are doing well. I just wanted to know how can I do dispacement controlled cyclic triaxial tests using ELDYN dynamic equipment. As you can see in the figure, there...

14 September 2023 2,217 0 View

How to find the contributions of electronic and vibrational?

generally polar basis set will provide the tensors (dipole moment,polarizability and hyperpolarizability). but how to find their contributions separately

24 June 2023 1,296 6 View

Why is cell conditioned medium concentrated after collection? For transplantation experiments, can it be used without concentrating it?

To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.

01 March 2023 695 3 View

Is there any model which is more suitable for contrastive learning except SimCLR and MOCOv2?

These two models take a lot of time to train with powerful GPUs and required a minimum 512 batch size for good model performance.

28 February 2023 1,654 1 View

How to calculate the depletion width (width of space charge layer) using Mott-Schottky analysis?

Width of depletion region which can be remarked using width of space charge layer (WSCL).

06 February 2023 2,621 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View

How can I put an alert to receive notifications of call for papers in my research field?

I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...

01 June 2024 1,352 0 View

How many percent of specific antibodies in serum after rabbit immunization ?

Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...

13 May 2024 2,828 0 View

What role do autoimmune disorders play in causing infertility?

how can immunological interventions potentially address these issues?

04 April 2024 4,951 1 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

How can I add weight to difference in correlation between 2 groups to also reflect the higher correlation values in addition to the difference?

I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...

11 March 2024 1,000 4 View