Most referred method ..

Development of a DTPA Soil Test for Zinc, Iron, Manganese, and Copper (SSSAJ,Vol. 42 No. 3, p. 421-428,doi:10.2136/sssaj1978.03615995004200030009x)

Abstract: A DTPA soil test was developed to identify near-neutral and calcareous soils with insufficient available Zn, Fe, Mn, or Cu for maximum yields of crops. The extractant consists of 0.005MDTPA (diethylenetriaminepentaacetic acid), 0.1M triethanolamine, and 0.01M CaCl2, with a pH of 7.3. The soil test consists of shaking 10 g of air-dry soil with 20 ml of extractant for 2 hours. The leachate is filtered, and Zn, Fe, Mn, and Cu are measured in the filtrate by atomic absorption spectrophotometry.

The soil test successfully separated 77 Colorado soils on the basis of crop response to Zn, Fe, and Mn fertilizers. Critical nutrient levels must be determined separately for each crop using standardized procedures for soil preparation, grinding, and extraction. The critical levels for corn using the procedures reported herein were: 0.8 ppm for Zn, 4.5 ppm for Fe, and tentatively 1.0 ppm for Mn, and 0.2 ppm for Cu.

Development of the soil test was based, in part, on theoretical considerations. The extractant is buffered at pH 7.30 and contains CaCl2 so that equilibrium with CaCO3 is established at a CO2 level about 10 times that of the atmosphere. Thus, the extractant precludes dissolution of CaCO3 and the release of occluded nutrients which are normally not available to plants. DTPA was selected as the chelating agent because it can effectively extract all four micronutrient metals. Factors such as pH, concentration of chelating agent, time of shaking, and temperature of extraction affect the amount of micronutrients extracted and were adjusted for maximum overall effectiveness.

Please see methodology given in the following paper:

http://www.scielo.br/pdf/sa/v67n1/v67n01a09.pdf

Dear David thhe available micronutrients Fe, Mn, Cu and Zn  in soil  extract with diethylenetriamine penta acetic acid and triethanolamine buffer (0.005 M DTPA+0.01 M CaC12+0.1 M TEA) according to (Lindsay and Norvell, 1978). 10 g air-dry soil (passing through a 2 mm sieve) weigh into a 125 ml Erlenmeyer flask,  add 20 ml extraction solution, shake for 2 hours and then filter the suspension. Flask containing all chemicals but without soil  include as blank. and read The concentration of available Fe, Mn, Cu and Zn through atomic absorption spectrometer by using appropriate lamp for each element. Extractable micronutrients cations in soil calculating by following formula: Fe,Zn,Cu or Mn ppm = (ppm in extract−blank) x A / Wt

Where: A= Total volume of the extract (ml) Wt= Weight of air dry soil sample ( g)

Most referred method ..

Development of a DTPA Soil Test for Zinc, Iron, Manganese, and Copper (SSSAJ,Vol. 42 No. 3, p. 421-428,doi:10.2136/sssaj1978.03615995004200030009x)

Abstract: A DTPA soil test was developed to identify near-neutral and calcareous soils with insufficient available Zn, Fe, Mn, or Cu for maximum yields of crops. The extractant consists of 0.005MDTPA (diethylenetriaminepentaacetic acid), 0.1M triethanolamine, and 0.01M CaCl2, with a pH of 7.3. The soil test consists of shaking 10 g of air-dry soil with 20 ml of extractant for 2 hours. The leachate is filtered, and Zn, Fe, Mn, and Cu are measured in the filtrate by atomic absorption spectrophotometry.

The soil test successfully separated 77 Colorado soils on the basis of crop response to Zn, Fe, and Mn fertilizers. Critical nutrient levels must be determined separately for each crop using standardized procedures for soil preparation, grinding, and extraction. The critical levels for corn using the procedures reported herein were: 0.8 ppm for Zn, 4.5 ppm for Fe, and tentatively 1.0 ppm for Mn, and 0.2 ppm for Cu.

Development of the soil test was based, in part, on theoretical considerations. The extractant is buffered at pH 7.30 and contains CaCl2 so that equilibrium with CaCO3 is established at a CO2 level about 10 times that of the atmosphere. Thus, the extractant precludes dissolution of CaCO3 and the release of occluded nutrients which are normally not available to plants. DTPA was selected as the chelating agent because it can effectively extract all four micronutrient metals. Factors such as pH, concentration of chelating agent, time of shaking, and temperature of extraction affect the amount of micronutrients extracted and were adjusted for maximum overall effectiveness.

Sir,

Pl see ref : Lindsay, W.L. and Norwell, W.A. 1969    Equilibrium relationships of

Zn2+, Fe3+, Ca2' and H+ with EDTA and DTPA in soils. Soil

Sci. Sot. Am. Proc. 33, 62-68

another ref :

I really appreciate you all for your answers. They're helpful.

Please don't assume that DTPA  = bioavailable

Yes I agree with you Paul. Of late, DTPA was subjected to great critisms in certain soils , it hardly related to croo response...