As a follow-up to my earlier question, the experiment i really love to conduct involves the use of Zn-65 isotope to trace the activity of manure-released Zn in plant. Please how (methodology) should the manure be labelled so as to easily differentiate Manure -Zn from soil- Zn in the result of my plant analysis.
Dear David,
Agricultural soils, and to evaluate the ability of chemical extraction methods to estimate plant-available soil Zn using isotopic techniques. The surface horizon (0–20 cm) of seven polluted and non-polluted soils representing a wide range in physico-chemical properties and Zn contents were sampled. An isotopic exchange kinetics (IEK) approach was used to assess, in a batch experiment, the isotopically exchangeable Zn content (E value). In order to determine the L values, a pot experiment was carried out with Lolium multiflorum (cv. Axis) in a growth chamber using a 65Zn-isotope dilution technique. Total Zn uptake and the isotopic composition (65Zn/stableZn) were determined in Lolium multiflorum for five successive cuts. The amounts of zinc extracted by different chemicals were compared with L values and regression parameters were estimated. The isotopic composition in soil extracted by DTPA and EDTAAc at the end of the pot experiment was also determined. Results showed that the equation describing the increase of isotopically exchangeable Zn with time could be extrapolated to three months for polluted and non-polluted neutral and acidic soils, and that the results were not different from the amount of isotopically exchangeable Zn experimentally determined with Lolium multiflorum (L value). In alkaline soils however, results suggest that either 65Zn sorption occurred in the batch experiment or that the concentration of Zn in the soil solution had been overestimated, leading to an overestimation of the E value compared to the L values. Furthermore, the specific activities measured in DTPA and EDTA extractions at the end of the pot experiment were significantly different compared to the specific activity of the plant, showing that both these chelating agents extract neither all the available soil Zn nor only the available soil Zn for plants.Zinc isotopes can be fractionated by a number of chemical, biological, and physical processes. This characteristic provides the opportunity to investigate the bio-inorganic chemistry and cycling of this micronutrient element in the environment
Regards,
Prem Baboo
https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwipi76O4Y3LAhVKH44KHTlaB9kQFgguMAI&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC2990013%2F&usg=AFQjCNFCZtnEFNLhn-Jtuc78Mm3pKGvByA&sig2=f6zivcovzD4KpxJRAwQXRg
- Badges
- Science topic
- Similar topics
- Agriculture
- Soil Science