I am working on moelcular characterization of Root knot nematode (Meloidogyne sp.) and facing some problem with quality of DNA. So please I request to send or suggest the best protocol for DNA extraction.
If you would lt me know/have the protocol you used previously as well as the particular challenge you are having, I might be able to help. I have previously worked on nematode nucleic acid extraction and realised that a number of factors can affect the quality of your nucliec acid.
Hi Tushar the paper I sent gives instructions for extraction from bulk eggs and also for PCR from a single J2 and references for further information. Hopefully this will help but get back to me if you still have a problem.