Pleas is it OK to select the primer sequence for Homosapiens Paraoxonase (PON1) gene to span only the exon-exon junction or leave it to no preference?

This is a hint from the NCBI database: This controls whether the primer should span an exon junction on your mRNA template. The option "Primer must span an exon-exon junction" will direct the...

11 January 2024 5,814 0 View