How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

What is a diagnostic resistance in malaria?

For malaria research experts/Scientists I am experiencing a full explanation of the following concept: I have been looking at the biological threat of malaria to the control efforts, where the...

16 October 2020 1,563 4 View

What is the status of malaria vaccine?

Malaria is the highest killer disease in Africa, yet no vaccine has been developed against it. Any hope in sight?

05 October 2020 5,670 7 View

Are 12-week-old C57 mice resistant to cerebral malaria?

14 September 2020 460 4 View

Hydroxychloroquine Protonation in Blood and Cells - inconsistent information on claimed alkalinization inside targets, emphasis on Coronavirus?

Some researchers have claimed that Hydroxychloroquine (HCQ) can enter cells and make the environment more "alkaline" by soaking up protons. Many publications discuss only 2 nitrogen atoms that can...

08 August 2020 6,262 3 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

What is the mechanism of action of HCQ+Azi against covid 19 infection?

how H CQ+Azi acts against covid 19?

31 March 2020 4,043 9 View

What important factors are to be considered to met the objectives of malaria elimination program?

Countries like Ethiopia has developed malaria elimination program. But, I am not sure how this program is implemented .

10 November 2019 2,585 4 View

Please how can I find the primers and the restriction enzyme for my SNP: CLEC4C rs12310416?

I am working on the association of CLEC4C rs12310416 polymorphism with clinical malaria in children in South Western Nigeria, however, I need a publication that has worked on this before so I can...

08 September 2019 6,272 4 View