Any protocol for mycoplasma plasminogen activation assay?
I have cloned a gene in pGEX-4T-1 expressed in JM109 and purified the recombinant GST-tagged protein using gluthathione sepharose bead column chromatography having confirmed the correct sequence....
01 February 2014 3,176 12 View
I obtained the EnzChek peptidase/protease assay kit. I carried out the assay according to the manufacturer's instruction using a microplate and read at excitation/emmission of 490/520 but i got...
31 December 2013 1,720 2 View
I have been trying to express a GST-tagged protein that was ligated into pGEX-4T-1 in JM109 cells. I have confirmed my plasmid to be in JM109 by PCR and digested out my insert and vector and they...
05 June 2013 3,664 9 View
What role do the ABC transporters play in the pathogenesis of mycoplasmas?
31 December 2012 697 1 View
What is the optimal OD600 and IPTG concentration for inducing E. coli cells for protein expression?
31 December 2012 3,327 20 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
