Any protocol for mycoplasma plasminogen activation assay?
I have cloned a gene in pGEX-4T-1 expressed in JM109 and purified the recombinant GST-tagged protein using gluthathione sepharose bead column chromatography having confirmed the correct sequence....
01 February 2014 3,310 12 View
I obtained the EnzChek peptidase/protease assay kit. I carried out the assay according to the manufacturer's instruction using a microplate and read at excitation/emmission of 490/520 but i got...
31 December 2013 1,828 2 View
I have been trying to express a GST-tagged protein that was ligated into pGEX-4T-1 in JM109 cells. I have confirmed my plasmid to be in JM109 by PCR and digested out my insert and vector and they...
05 June 2013 3,783 9 View
What role do the ABC transporters play in the pathogenesis of mycoplasmas?
31 December 2012 798 1 View
What is the optimal OD600 and IPTG concentration for inducing E. coli cells for protein expression?
31 December 2012 3,874 20 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
