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Questions related from Adamu James Yazah
I have cloned a gene in pGEX-4T-1 expressed in JM109 and purified the recombinant GST-tagged protein using gluthathione sepharose bead column chromatography having confirmed the correct sequence....
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I obtained the EnzChek peptidase/protease assay kit. I carried out the assay according to the manufacturer's instruction using a microplate and read at excitation/emmission of 490/520 but i got...
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I have been trying to express a GST-tagged protein that was ligated into pGEX-4T-1 in JM109 cells. I have confirmed my plasmid to be in JM109 by PCR and digested out my insert and vector and they...
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What is the optimal OD600 and IPTG concentration for inducing E. coli cells for protein expression?
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Any protocol for mycoplasma plasminogen activation assay?
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What role do the ABC transporters play in the pathogenesis of mycoplasmas?
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