6 Questions 13 Answers 0 Followers
Questions related from Adamu James Yazah
I have cloned a gene in pGEX-4T-1 expressed in JM109 and purified the recombinant GST-tagged protein using gluthathione sepharose bead column chromatography having confirmed the correct sequence....
02 February 2014 3,266 12 View
I obtained the EnzChek peptidase/protease assay kit. I carried out the assay according to the manufacturer's instruction using a microplate and read at excitation/emmission of 490/520 but i got...
01 January 2014 1,794 2 View
I have been trying to express a GST-tagged protein that was ligated into pGEX-4T-1 in JM109 cells. I have confirmed my plasmid to be in JM109 by PCR and digested out my insert and vector and they...
06 June 2013 3,746 9 View
What is the optimal OD600 and IPTG concentration for inducing E. coli cells for protein expression?
01 January 2013 3,672 20 View
Any protocol for mycoplasma plasminogen activation assay?
01 January 2013 3,253 0 View
What role do the ABC transporters play in the pathogenesis of mycoplasmas?
01 January 2013 762 1 View
