19 July 2018 5 127 Report

Hello all,

Excuse my ignorance but I was looking on addgene for some high fidelity Cas9 nucleases, combined with some selection tools (i.e. GFP and an antibiotic), however most that I found seemed to have no insert site for the sgRNA template. Is it just assumed that you will insert a second plasmid with the sgRNA scaffold?

Any advice would be much appreciated

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