Hello everybody,
I generated DNA sequence of a specific marker of an endemic plant species. When doing BLAST search, I realized that the marker I selected had not been sequenced before.
The question is, how to use this novel sequence as DNA barcode without reference sequences to be compared?
Thank you for answering me
All the best
We spent also a lot of time in identifying plant species. try out BOLD instead of BLAST as there are more specific sequences in the plant specific database. It includes also variouse marker genes. Of course the BLAST sequences were included in that database but there are more sequences in the BOLD project database
You may also search the sequence in BOLD database and submit your sequence. BOLD system will generate the Barcode for respective plant species
Hi]here you can submit your confirmed sequence to NCBI as your sequence for this gene and its published online
When doing BLAST search for a sequence which has not yet been added to the library of barcodes, and not included in the dataset (BLAST or BOLD), you won't have a match. You won't have reference sequences to compare it against. You need to submit your sequence and publish it as a barcode for that endemic species.
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