Hi! I'm planning on using methyl-3-nitro-1-nitrosoguanidine (MMNG), for inducing transcriptional mutagenesis. From my understanding MNNG causes the formation of O6‐methyl guanine (O6MeG) inducing thymine mispairing during DNA replication. However, is it likely to for MMNG to to lead to other mispairing that may occur in low abundance? Or are there any disadvantages to be mindful of? I want to ensure that I can accurately quantify mutation patterns.
Thank you for your insight :)
No alkylating agent (with the possible exception of those containing a targeting moiety) can be considered selective it is more of a general preference (see Coles, B. Effects of modifying structure on electrophilic reactions with nucleophiles. Drug Metab. Rev. 1985, 15, 1307−1334.) MNNG in addition to targeting the O-6 position of guanine, will cause depurination, strand breaks, alkylate phosphate etc. etc. For a more detailed discussion see Article Antitumor Sulfonylhydrazines: Design, Structure–Activity Rel...
MNNG will do what you seek, but you must be aware of it's other targets, which are not restricted to the O-6 position of guanine in DNA, or even nucleic acids, or macro molecules.
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