Pittsburgh Sleep Quality Index Scoring Synthax on Excel?

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For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

Procedure of Ammonium sulfate precipitation of ACE2 in Calu-3 cells?

Hello, I am trying to find a protocol that looks at the extraction and purification of ACE2 inside the cell. We have the protocol for the affinity chromatography, but we are having trouble...

23 May 2024 4,342 1 View

Can anyone identify these Cumaceans?

Hi everyone, I've been struggling to identify the family these guys belong to. Does anyone have any ideas? Taken from NE Atlantic 2200m depth.

23 April 2024 1,282 4 View

When washing demineralized fish scales, should I be concerned when there are string like substances (most probably fibers) are present?

My study is about synthesizing fish scales into chitosan and I am currently washing my demineralized fish scales. My sample fish scales were size reduced beforehand and when 1% HCl was added, it...

16 April 2024 2,129 0 View

In assessing the viability of using lithium heparin from gel separatory serum, what other tests can be added as parameters to further the study?

In the present day, countless tests are being run in the clinical laboratory. In the Journal of Oguzhan Zengi entitled, "The Transition from Gel Separatory Tubes to Lithium Heparin Gel Tubes in...

10 April 2024 3,541 1 View

How to make a thesis introduction?

how to start my thesis introduction

24 March 2024 2,366 1 View

What are some economical methods which can be employed by tertiary hospitals to develop & utilize regional antibiograms in the Philippines?

In the Philippines, national databases on antibiotic resistance are noted through the Department of Health (DOH) Antimicrobial Resistance Surveillance Program (ARSP). However, community or...

29 February 2024 4,600 1 View

What's wrong with Oil red O staining of the liver slices?

Hi everyone, the attached picture is oil red o staining of mouse liver slices after GAN diet, which was to induce NASH. I am wondering what are those round empty space with the clear boundary? Are...

27 February 2024 5,743 0 View

Fixed brain in 30% sucrose solution froze at 4°C?

I've been trying to figure out what happened but it seems I am the only one this has happened to? Processing: - transcardial perfusion with 1x PBS followed by 4% PFA - post-fix brain in 4% PFA...

12 February 2024 5,963 2 View

Sharing link to ResearchGate on LinkedIn?

is It possible to share my ResearchGate profile on LinkedIn so I only need to update one of them? Thanks!

28 October 2023 4,675 0 View

Why do men not accept that continually hassling for sex proves that they want it more than their partner?

Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...

08 August 2024 1,491 0 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

I am considering injecting cisplatin directly into an equine sarcoid tumour that is chronically refractive?

My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...

05 August 2024 1,835 0 View

How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

30 July 2024 1,607 1 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

Medical question: Is OSA the cause of Afib and HTN?

Does Obstructive Sleep Apnea either cause or exacerbate Atrial fibrillation? Hypertension? We know they are comorbid factors in 50-70% of all OSA patients, and with OSA being so hard to detect...

29 July 2024 7,760 1 View

I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?

27 July 2024 5,916 1 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

25 July 2024 6,242 2 View

How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View