Picrosirius red staining without picric acid?

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Weird bands in my SDS PAGE gel : how can I resolve this issue ?

Hi ! I am trying to run some gels but everytime I do, my samples end up looking very wavy while the scale is ok. For now my protocol is : sds page gel 12% acrylamide, run at 200V at room...

10 April 2024 4,873 4 View

NVIVO 12 (for Mac) - How to view number of added nodes for later interviews?

I am undertaking my first research study and getting to grips with NVIVO 12. I have completed initial coding for three interviews so far and have realised that I haven't kept note of how many new...

27 February 2024 8,184 4 View

Are you looking for a full-time academic research job in Hong Kong (Postdoctoral Fellowship in Sociology, The Hong Kong Polytechnic University)?

Postdoctoral Fellowship in Sociology Department of Applied Social Sciences, The Hong Kong Polytechnic University Salary and benefits: highly competitive (commensurate with qualifications and...

06 February 2024 7,266 1 View

What strategies should we implement in retaining the regular safe blood donors in countries where transfusion-transmitted infections are prevalent?

According to WHO (2012), blood donor selection is the first crucial step in the process of ensuring blood safety as it helps to significantly reduce risk of any individuals or groups of...

01 September 2023 2,353 0 View

Can I dissolve collagen IV powder in PBS for flask coating?

Hi, I purchased collagen IV powder from Sigma and the website says to dissolve in acetic acid, PBS or water. As I didn't have acetic acid I tried to dissolve it to 1mg/mL in PBS but the collagen...

05 July 2023 3,420 1 View

Please, can you suggest me authors with papers, articles, dessertations, essays, etc., about St. Johns pottery in Florida?

I need more information about sherd analysis that belongs to the St. Johns Series, the spiculae sponge resources origin in Florida, the possibility of establishing a comparative analysis of these...

30 June 2023 4,060 2 View

How to justify chosen age range in recruitment sample for dissertation?

Hi all, I am completing my undergraduate dissertation in Sociology, looking at female university students age 18-23. My questions seeks to answer why women choose 'feminine' degree choices...

07 April 2023 1,938 2 View

Does anyone know if there is any method to quantify an ELISA test if I do not have a standard of a known concentration?

I have a commercial qualitative ELISA KIT, but it does not have a standard, there is no commercial standard for this protein, and I need a quantitative estimation. Does anyone have an idea?

08 March 2023 8,175 4 View

Visualize interaction effects in spatial regression?

I am trying to create interaction plots to visualize the interaction effects in a spatial error model. I created the attached charts for a linear regression model in R, but the packages I used to...

28 October 2022 7,483 1 View

How to delimit area to calculate UHI?

To calculate the UHI, is it better to delimit only the urban area (work with that built up area) or should I work with a larger section that includes the urban area and also the rural areas? I ask...

22 October 2022 2,657 9 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View