Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Dopamine transporter antibody?

I am looking for an antibody against dopamine transporter suitable for WB. I have tried a couple of antibodies but I obtained poor results. Can anyone help me?

18 May 2020 1,536 2 View

Experience with siRNA transfection of neural stem cells?

Does anyone have experience in gene silencing through siRNA transfection specifically in neural stem cells (ReN cells)? I know these are hard to transfect cells, and with other methods I normally...

29 March 2020 4,210 0 View

Can anybody help me with Boyden chamber invasion assay for HCT-116 cell lines?

I have a problems with Boyden chamber assay for HCT-116 cell lines, I did not see any stained cell in the membrane. I have had a success with other cell line, A549. Does anyone have any...

23 March 2020 7,119 5 View

How to make cell distribute evenly in 12-well plate?

I worked with cell culture 1 year ago, nearly I have a problem when I seeding in 12-well plate, more cells settles in the middle of well. I am trying with different 'shaking method'. I hope to get...

19 March 2020 9,999 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

Can anyone recommend any strong papers concerning the prevalence of PTSD and/or CPTSD in paramedics?

Dear all, I am in the early stages of undertaking research surrounding PTSD/CPTSD and the prevalence of these conditions in paramedics. Can anyone point me towards some recent research in this...

29 October 2019 8,117 1 View

What is the best way to isolate the full leukocyte fraction from mouse blood?

I would like both monocytes, lymfocytes and granulocytes to eventually stain and stick on a slide. I used lymphoprep before, but this only gives me the mononuclear cells. I would like the complete...

08 October 2019 1,717 3 View

Could someone guide me towards my hesitation to choose my tax law thesis topic?

Good afternoon from Peru! I'm currently working on my thesis (Tax Law field), but I can't seem to decide which topic I would like to explore, because I am really interested in this three...

20 June 2019 4,419 4 View

Why is my whole western blot gray?

I have attached a picture for my most recent western blot. My Beta-actin always shows up completely perfect but all other protein blots appear gray and messy. This has happened multiple times....

31 May 2019 5,364 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View