Hello. Hopefully, everyone is doing well. I usually use the Pichia protein expression system to express my recombinant protein. Every time working with this system was so easy for me and had no problem at all but for the last two months whenever I try to express the same proteins in this system, after purification i notice lots of DNA contamination that cannot be removed from my protein sample. I want to know if anyone faced the same problem before as I do not understand what has been changed in my procedures which make me face this huge problem. If anything you can mention which may help me is really appreciated. (I also tried so many wayed to get rid of this contaminant but was not useful) THANKS
Is your expressed protein a transcription factor or DNA binding protein?
Have you tried doing a DNA digest step?
Robert Adolf Brinzer Hello, and thanks for your response, dear Robert. My protein is not a binding DNA protein and using DNase is exactly the method I am thinking about these days. Could you please take a look at these two brands and tell me if they are okay to buy and give a try?
1: https://international.neb.com/products/m0303-dnase-i-rnase-free#Product%20Information_Reagents%20Supplied
2: https://www.genscript.com/enzyme/E00053-DNase_I.html
Thanks in advance for your great help.
NEB tends to be consistent with the quality of their products. Never used Genscript before so cannot give an opinion.
Robert Adolf Brinzer Dear Robert I can use this NEB DNase for my purpose.
Could you please take a look at this product and let me know your idea? Already bought one DNase but was not good enough to do this experiment. Thanks in advance
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