Majid Mousavi

6 Questions 7 Answers 0 Followers

Questions related from Majid Mousavi

Pichia protein expression system (Yeast) DNA contamination?

Hello. Hopefully, everyone is doing well. I usually use the Pichia protein expression system to express my recombinant protein. Every time working with this system was so easy for me and had no...

23 August 2023 7,499 4 View

How to remove DNA contamination?

Hello. Hope everyone is doing well. i purified my protein with the Q column (ion exchange column). The first time everything was ok but when I tried for the second-third time i got lots of DNA...

24 June 2023 2,341 4 View

Pichia expression system needs Histidine in order to grow?

My question is about the function of Histidine in the medium. I know there is a mutation in our pichia cell line and we need to transform our plasmid into the host cells and make it able to...

18 December 2021 3,225 1 View

How to add a sequence about 70bp to a plasmid?

i have plan to add a secretion signal to my plasmid. the point is here that i do not have any source to PCR that sequence and at last do GA. i need to synthesize by myself. i want to know is it...

04 September 2021 6,376 3 View

Hello guys. Im confused about how secondary structures can bother cloning procedures? Does anyone know what is the logic behind issue??

Im doing cloning using GA but i failed two times and i found the maybe it can be due to secondary structure in my DNA. I wanna know how these secondary structures can disturb our cloning...

01 June 2020 7,991 0 View

Hello.Im going to freeze my SF9 and HI5 cells.I wanna know whether i can use the same freezing medium for both of them or not and how to make it?

Actually i want to know how to make freezing medium with 10%DMSO& 90% FBS?What should be the condition to keep it and how long time is the shelf-life?Much tnx for your suggestions.

21 May 2019 7,509 3 View