Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

Methanol usage in determination of vitamin B2 in foods using HPLC?

After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...

16 January 2021 3,740 4 View

How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

How to bind streptavidin to the surface of acrylamide?

We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!

09 December 2020 8,141 7 View

Can we do electrophoresis of RNA from baker's yeast by vertical electrophoresis (using acrylamide gels)?

I want to do an electrophoresis of RNA from baker's yeast. However, our lab only has a vertical electrophoresis, while usually electrophoresis of nucleic acid (RNA or DNA) use horizontal...

29 November 2020 7,938 5 View

Can anyone help or advise how and by what mechanism graphene oxide-activated persulfate can react with acrylamide and polyacrylamide in water solvent?

What are the products of the reactions mentioned above? And by what mechanism are they produced? Does graphene oxide itself participate in the reaction? Does not sulfate react with graphene...

18 November 2020 6,938 6 View

Can i change buffer pH without changing precast gel pH?

Can i run a precast TBE native PAGE gel (made with tris pH 8.3) with 1x TBE running buffer of lower pH such as 7.0? Would the lower pH of the running buffer lower the gel pH if i pre-run the gel...

11 November 2020 3,235 4 View

Hi everyone, Can anyone kindly help me for analyzing the attached FTIR spectra?

It's an organic material used as a dispersant for refractory concretes. I had some search and found that it can be an acrylamide based compound but I am not sure yet. The datasheet supplied by the...

04 October 2020 994 9 View

Why my gels got different distributes when I change acrylamide?

Hi researcher and fellows, the protein bands separated differently as compared in two gels (12.5%). The sample for both is the same but it looks like weird in the second gel. That gel did after I...

28 March 2020 7,842 4 View

PCR-DGGE problem, no bands appear in certain lanes?

I extracted DNA from several bacterial isolates and I amplified the V1-V3 region of the 16S rRNA gene of these samples. However, when I ran a DGGE with these samples, several lanes did not contain...

23 March 2020 2,914 8 View