Phosphorothioate CpG ODN as an oral adjuvant for delivery of recombinant protein antigen in poultry

More Adarsh Mishra's questions See All

Is it possible to get unprocessed extracellular recombinant protein during yeast expression?

Is there any chance of getting protein with signal sequence without processing by Kex2 or Ste13 signal cleavage, while expressing our target protein in pPICZ-alpha vector in Pichia pastoris GS115?

02 March 2017 3,880 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

Laith Al-Ani

Hi Mishar

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059049/

https://www.tandfonline.com/doi/full/10.1080/03079457.2011.640305

https://link.springer.com/content/pdf/10.1385%2F1-59745-168-1%3A139.pdf

https://cvi.asm.org/content/21/3/329.full

https://iai.asm.org/content/69/8/4816.full

https://naldc.nal.usda.gov/download/38735/PDF

http://orbit.dtu.dk/files/5295844/Adjuvants_and_delivery_systems_in_veterinary_vaccinology_REVISED2.pdf

https://www.jimmunol.org/content/160/8/3627.abstract

https://www.pnas.org/content/109/14/E797.long

http://salem.lab.uiowa.edu/sites/salem.lab.uiowa.edu/files/Salem%20-%20Advanced%20Drug%20Delivery%20Reviews%202009%20Review_1.pdf

https://www.crcnetbase.com/doi/pdf/10.3109/9781420060744-27

https://www.pressesagro.be/base/text/v17n3/483.pdf

https://www.google.fr/patents/US7488490

https://www.mdpi.com/2076-393X/3/1/148/pdf

https://icmr.nic.in/ijmr/2013/November/22.pdf

http://www.nature.com/nri/journal/v4/n4/full/nri1329.html

https://www.hindawi.com/journals/bmri/2013/636847/

http://www.academia.edu/21434095/Toll-like_receptor-based_adjuvants_enhancing_the_immune_response_to_vaccines_against_infectious_diseases_of_chicken

https://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2002.tb00551.x/full

https://online.liebertpub.com/doi/full/10.1089/vim.2015.0144