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How to Calculate Flow Rate Ratio (FRR) for a Three-Inlet Microfluidic Chip BASED ON Hydrodynamic Focusing?
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I need help calculating the encapsulation efficiency of lipid nanoparticles loaded with Fam-labeled DNA using a microplate reader. I am using ultracentrifuge filters to separate the unloaded DNA....
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I need help using a microplate reader to measure the intensity of a fluorescent dye. Could you provide any recommendations and instructions for doing this?
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What is the effect of the depth of a microfluidic chip based on hydrodynamic focusing on liposome particle size?
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Which is easier to use for observing DNA oligonucleotides on AFM: a fluid cell or a mica substrate?
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I am fabricating a PDMS microfluidic channel with a depth of 100 µm and a width of 140 µm in a T-shape. However, I am encountering backflow issues when I introduce my sample. Is there any way to...
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I'm trying to cleave mica with a blade, but it breaks (it's brittle) before I can cleave it. Is there a specific way to achieve a clean cut with mica?
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What is the best preparation method for characterizing liposomes in PBS solution using FTIR?
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Can I use a glass substrate rather than mica to characterize liposomes in AFM?
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I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
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How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
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I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
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How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
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What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...
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