Hi, I am performing a phagocytosis assay of monocytes derived macrophages on primary hepatocytes (mouse).
the hepatocytes are isolated from normal liver. I would like to know if I should rely on “basal” “eat me signals” or is it better to induce apoptosis by UV irradiation (of target hepatocytes), to get a both a strong and repeatable output.
I guess the basal eat me depends on cell stress and % of dying cells which can change between experiments.