Can an airplane stay up in the the air without moving forward just like helicopter ?

How the lift and weight cancel each other perfectly for a helicopter to stay it motionless? Because its propeller do generate huge amount of lift due to air pressure plus a blend of air stream...

09 February 2021 3,990 14 View

How to take p-xrd sodiated carbon anode material?

Can i collect p-xrd data of sodiated carbon anode of sodium ion battery in presence of air? Or I have to do the xrd in inert atmosphere?

23 January 2021 3,506 2 View

What is the formula used for the calculation of L10?

Is there any formula, which can be used for the calculation of L10 i.e, peak noise level? Please give your opinion?

14 January 2021 8,408 3 View

How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

How do moisture and air contribute to the capcity fading of fabricated anode materials for Na-ion batteries?

I am using a glove box with N2 supply for Na ion battery coin cell assembly. But the oxygen level is more than 20 ppm. Can anyone one suggest whether 20ppm oxygen level is okay for battery...

15 November 2020 5,727 3 View

Probe based primers for conventional PCR?

27 August 2020 1,935 2 View

While doing NIH quality assesement of cross sectional/ cohort studies ?

for e.g. lets say, out of 14 question, if we give 1 point to 3 question , 0 point to 1 study, other 10 questions are "not applicable" to this study. what is total score and what is conclusion

13 April 2020 7,574 2 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

08 March 2020 9,612 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

06 January 2020 3,767 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View