Can somebody suggest sample preparation protocol for FT-IR and SEM analysis for Inclusion body protein?

I want to do structural characterization of my recombinant protein which is going into inclusion body

15 January 2020 6,762 1 View

From where can I procure colloidal carbon nanoparticles in India?

I am aiming to use carbon nanoparticles in my immunochromatography assay & most of the reference paper have use Colloidal carbon particles ( Spezial Schwartz 4 [SS4]; Degussa AG, Frankfurt,...

26 March 2019 9,077 3 View

What are the best practices to model structure of an insect olfactory / odorant receptor (computationally) according to you?

Kindly suggest your views on the topic. Please provide references wherever possible.

18 August 2017 5,106 7 View

What exactly is the concept of antibody pair with respect to lateral flow assay?

30 July 2017 4,972 3 View

What are the markers to identify higher milk secretion in the mammary glands of lactating Wistar rats?

I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the...

21 March 2017 3,172 1 View

How can we determine the molecular weight of an inorganic polymer?

I want to determine molecular weight of poly aluminum chloride.

01 May 2014 3,760 2 View

Fermentation technology involved with product processing.

I need some research resources on downstream prossesing purification & pakaging, drying,recovery of fermentation products.

26 February 2012 7,230 12 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View