pET32b for protein expression?

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What is the Standard Procedure for measuring Total Lipid concentration in Daphnia magna?

currently working on a project involving Daphnia magna and I am intertied in measuring their total lipid concertation. I was hoping to get some insights or advice on the standard procurers for...

23 May 2024 8,846 1 View

What is 1335 in acetyl value and why this value put here as constant?

what is 1335 in acetyl value and why this value put here as constant? what is 1335 in acetyl value and why this value put here as constant?

10 January 2024 8,357 0 View

How to decide the practical applicability of our prepared super-capacitor electrode material for specific application in portable electronics?

Hello, Researchgate community, I have one query regarding deciding whether our electrode material is suitable for practical energy storage applications. When we prepare and characterize...

22 October 2023 6,027 3 View

How to decide the concentration need to induce oxidative stress in isolated mitochondria of rat brain by using either rotenone & sodium nitroprusside?

I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...

11 June 2023 6,247 2 View

How to calculate the GSH present in mitochondrial protein in umol/gm?

While doing GSH on rat mitochondria, I have calculated protein concentration by Bradford's assay (1.35mg in 2ml buffer). In case of GSH assay I got concentration of 0.23mM (GSH) in mitochondria....

03 May 2023 2,820 6 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

07 February 2023 2,865 0 View

What is the Methodology for calibration curve of rotenone?

Rotenone is the insecticide used as ROS Inducer in mitochondria. In aiming that what will be the method for the preparation of calibration curve for rotenone?

07 February 2023 5,471 0 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

07 February 2023 6,362 4 View

Is tag removal required from fusion protein?

I have a thioredoxin tag with my therapeutic protein. I have purified it using Ni-NTA. Next, I want to check it's activity by Elisa or cell proliferation assay. Is it necessary to remove the tag...

11 November 2021 6,284 10 View

How to achieve biotinylation of polyclonal antibodies?

I want to biotinylate polyclonal antibodies. The application of these polyclonal antibodies would be in the lateral flow assay. I am not looking for any commercial kit. Please help with the...

23 September 2021 8,241 5 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS?

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...

22 July 2024 5,334 6 View