What will be the percentage of my agarose gel , while running V3 region of 16SrDNA gene ?
Dear Dasgupta,
1.5% of agrose gel for 16SrDNA works good.
what percent of agrose have you used for the genomic DNA? for 16sRNA the concentration of agrose will be higher then the one used for your genomic DNA.
good luck
Thank you all for your valuable suggestions
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