Percentage of agarose gel ?

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Why am I observing a m/z peak at 372 for the ciprofloxacin hydrochloride hydrate spectra in MS?

The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.

01 June 2024 7,419 0 View

How to increase the short circuit current density (Jsc) in a Cs0.15FA0.85Pbi3 perovskite photodetector?

Hello guys, I am optimizing a perovskite photodetector device with the device structure ITO/PTAA/Cs0.15FA0.85PbI3/PCBM/BCP/Ag. PTAA (2 mg/mL): 5000 rpm/30 sec Perovskite (1.3 M): 1000 rpm/10...

10 May 2024 2,297 0 View

Hello guys, What might be the reason for getting two PL peaks after deposition of PCBM onto Cs0.15FA0.85PbI3 perovskite?

18 April 2024 5,169 0 View

How do I compare community between two samples which have different sample sizes?

I have data for two communities. The sample sizes are different. I wish to see if there is a difference between these two communities (each in a different location, hence the need for analysis) in...

06 April 2024 7,651 0 View

What is my research gate score what is my hi-index?

Research Interest Score 2,143 Citations 3,191 h-index

15 March 2024 9,688 1 View

How to increase Jsc in Cs0.15FA0.85PbI3 perovskite ?

Dear all, My device structure is ITO/PEDOT:PSS/Cs0.15FA0.85PbI3/PCBM/PEI/Ag. But till now, I am getting Jsc nearly 13. So please suggest to me how I can increase my Jsc and why I can not achieve...

13 March 2024 9,601 1 View

Where do I get Functional Trait data for Indian birds?

Hello. I am looking for functional trait data for Indian birds. Does a database exist for the same? If so, could someone provide me with the link to the same? Thank you

01 February 2024 183 1 View

How to change the colour of each cell in a grid in arcmap?

Hello. I have an ArcMap file which has a layer with my study area and a numbered grid overlayed on it. Both are in the same layer. I wish to colour code each cell on the basis of the built up area...

05 December 2023 8,127 1 View

Can anyone tell me the probable reasons of the occurrence of another peak(or a hump?) at around 1.3V? Should I consider both for further study?

I am studying the voltammetric detection of the synthetic food colour Amaranth (AMR). Different literature shows that the oxidation peak current appears at around 0.8V in the case of Cyclic...

03 December 2023 1,860 3 View

Can anyone guide me for the analysis of this FTIR plot of Y2O3 nanoparticles?

Can anyone guide me in the analysis of this FTIR plot of Y2O3 nanoparticles? please refer to some articles as references to this.

19 November 2023 7,153 5 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View