Himanshu Sharma

7 Questions 11 Answers 0 Followers

Questions related from Himanshu Sharma

Why is it that some crude protein are less precipitated with ammonium sulphate salts but readily precipitated with prechilled ethanol ?

I have been working on alpha amylase and tried precipitating it with ammonium sulphate salt. With ammonium sulphate salt i do get precipitates but they are flaky, float in the supernatant and...

23 July 2017 4,006 6 View

How to prepare glucose, mannose, sucrose, lactose, starch, ribulose, trehalose, mannose, sample for thin layer chromatography?

I need to prepare standard with sugars for which i need to know the solvent in which i should dissolve sugars if i will be using chloroform:acetone:water 6:7:1 ratio as solvent and spray of...

07 June 2016 8,240 2 View

Having problem with the recovery of protein when using large quantity of sample?

i am working on bacterial amylase and trying to precipitate protein by 1:7 sample and ethanol method.when i precipitate the small quantity of protein in one tube(in one eppendorf i.e.125ul sample...

12 February 2016 1,297 5 View

Which protein membrane centrifuge tubes should be used to concentrate the protein of 66 KDa?

i have precipitated protein using ethanol........do i need to concentrate the protein before i start chromatography techniques......... if yes then what centrifuge membrane tubes should i use for...

27 December 2015 836 6 View

How to speed up the destaining of sds page?

i am using sds page to know the molecular weight of protein i am working with. i have used coomassie blue to stain the gel. Its been over a week now that i am changing the destaining solution...

19 December 2015 3,839 4 View

How do I calculate the enzyme activity and specific activity?

i have prepared glucose standard graph and protein standard graphs and have obtained the mg/ml of glucose and BSA for 1 OD.. simultaniously i have done the enzyme assay and protein estimation.now...

24 October 2015 2,882 7 View

Why is my ammonium sulphate protein precipitation not working properly?

I started with partial purification of amylase using ammonium precipitation technique.While going for step by step saturation the recovery of protein was less than 10% of the crude amylase.So i...

06 October 2015 8,704 17 View