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Questions related from Himanshu Sharma
I have been working on alpha amylase and tried precipitating it with ammonium sulphate salt. With ammonium sulphate salt i do get precipitates but they are flaky, float in the supernatant and...
23 July 2017 3,970 6 View
I need to prepare standard with sugars for which i need to know the solvent in which i should dissolve sugars if i will be using chloroform:acetone:water 6:7:1 ratio as solvent and spray of...
07 June 2016 8,211 2 View
i am working on bacterial amylase and trying to precipitate protein by 1:7 sample and ethanol method.when i precipitate the small quantity of protein in one tube(in one eppendorf i.e.125ul sample...
12 February 2016 1,269 5 View
i am using sds page to know the molecular weight of protein i am working with. i have used coomassie blue to stain the gel. Its been over a week now that i am changing the destaining solution...
19 December 2015 3,803 4 View
i have prepared glucose standard graph and protein standard graphs and have obtained the mg/ml of glucose and BSA for 1 OD.. simultaniously i have done the enzyme assay and protein estimation.now...
24 October 2015 2,843 7 View
I started with partial purification of amylase using ammonium precipitation technique.While going for step by step saturation the recovery of protein was less than 10% of the crude amylase.So i...
06 October 2015 8,675 17 View