How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

Statistical Test for Single Group over Multiple Conditions & Different Time Lengths?

I have a dataset with an unusual configuration and was hoping for guidance on choosing a method to test for changes in mean. For context, this is operational data tracking vehicle arrivals at 10...

20 January 2021 4,429 4 View

How long does MQAE solution last once prepared?

I am wanting to prepare a small quantity of MQAE solution. Once prepared, how soon should it be used? How fast will it degrade? Is there a way I can store a leftover amount for future use? Are...

20 December 2020 1,416 3 View

Why do many stock buffers ask you to adjust pH and then add more water?

Could someone please explain why many stock buffers ask you to adjust the solution's pH and subsequently increase the volume to a set amount? For example: Add 500g of powder X to 800mL of...

09 December 2020 1,882 3 View

How to estimate sample size when you think the effect size will be reduced (compared to previous studies)?

Hi, I am planning a follow-up experiment to a previous study and have a question about estimating sample size: For the sake of simplicity, the previous study showed that Condition 1 had more...

19 November 2020 7,948 6 View

What is angular velocity of projection of uniform circular motion?

12 November 2020 481 5 View

How do crush foraminifera for d18O analysis?

I am looking to combine and crush 5 foraminifera to create a homogeneous carbonate powder that I will then be sending out for oxygen analysis. Does anyone have any good methods to make these...

14 October 2020 8,128 7 View

Troubleshooting co-IP from a small amount of brain tissue?

I am looking to perform a co-IP; however, I am using a very small amount of tissue to begin with from region specific microdissection in mice (2-3 300um sections per animal). I am concerned that...

07 October 2020 8,937 3 View

Can Stimulus Packages safeguard businesses during the Covid-19 Pandemic?

Research Proposals

02 October 2020 6,404 9 View

Magnetite ultrafine powder supplier?

Looking for a supplier that has 25 micron max particle size as an available option. Found one, but they won't even sell us a sample because they don't think it will work for us. Crazy! All I...

24 August 2020 2,752 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View