Anamika Rawat

4 Questions 8 Answers 0 Followers

Questions related from Anamika Rawat

How to perform Cre/lox recombination?

Hello, I would like to remove selection cassette from my mutant plant in order to transform another construct with same selection. I know, cre/lox recombination is one of the possibility, but...

10 October 2016 2,613 7 View

Monopteros gene in lower plants?

Hi, is there Monopteros gene present in lower plants like marchantia or physcomitrella patens? Any references would be just perfect. Thanks

08 August 2016 9,191 3 View

People working with Marchantia, Gamborg medium from Sigma or Duchefa, which one is better for Marchantia culture, any recommendation?

I am completely new in field of Marchantia! Would like to grow marchantia on Gamborg media. What is widely used for this purpose, confused between sigma and duchefa?

07 July 2016 9,164 6 View

How to purify a high molecular weight DNA fragment (>10kb) from agarose gel?

Needs some expert advice in purifying a 10,300 kb fragment (after restriction) from agarose gel? I am getting very low yield, only few ngs!!! whereas my starting concentrations is more than 5 ugs....

02 February 2016 8,754 8 View