TGF-Beta R1 inhibitor in Osteosarcoma?

Dear fellow researchers: I am working on osteosarcoma cells (U2OS) and am looking for ways to block TGF-Beta signaling pathway (towards Oct-4). Has anyone tried anything related to this...

07 August 2019 4,253 6 View

How to prepare 1.6% chromic acid?

Dear fellow researchers: I need to prepare 1.6% chromic acid for the purpose of AAO etching. The amount of information available online to make chromic acid is overwhelming. I also could not find...

09 October 2018 627 2 View

Cells not uniformly stained in FITC-Phalloidin?

Dear fellow researchers: Ive been doing this experiments for a while but recently I've observed that not all of my cells are getting stained with FITC-Phalloidin. Can anyone suggest possible...

08 September 2018 6,120 9 View

Recommended company to buy TGF-B1 antibody for IF?

Dear fellow researchers, I want to stain TGF-B1 in the cells. I haven't done this in the past. If some has, can anyone recommend a good primary Ab company (tried and tested) with good specific...

07 August 2018 6,637 5 View

No title

Dear Fellow Researchers, I am working on the anodization process and for a brief period of time (3 minutes), I need to achieve and maintain the temperature of the solution (oxalic acid) between...

07 August 2018 8,303 2 View

Why DC Power supply automatically switches from CV to CC while Anodization?

Dear fellow scientists: I am working on fabrication of nonporous Anodised Aluminum Oxide. I am performing the electrolysis in 0.3M oxalic acid at 40V (CV mode in the power supply). However,...

07 August 2018 7,040 6 View

Why the Binding affinity of organic compound with different chirality with FBS is different?

Dear all, I have an organic compound with the difference in chirality. The binding of R-chiralty with Fetal Bovine Serum (FBS) is lower than the binding of S-Chirality with FBS. While I have...

02 March 2018 9,040 12 View

How to perform SRB Assay for cells on opaque substrate?

Dear fellow researchers, I am aware of the SRB assay for analyzing viability of cells cultured directly on-to culture dishes. However, my cells are growing on opaque silicon substrates and I want...

02 March 2018 3,593 3 View

How to measure cellular adhesion to a substrate?

Dear fellow researchers, I'll be running some experiments where I will have to analyze the cellular adhesion to different substrates. Can someone recommend a specific technique to quantify the...

31 December 2017 766 9 View

Why study expression of phosphorylated genes?

Dear all, I have seen in many research papers that scientists study the expression of a gene in the modified stated as well as in the phosphorylated state after a specific treatment. Can anyone...

06 July 2017 5,166 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View