I need to detect Pseudomonas syringae pv. actinidiae in symptomless plant material. What is the best PCR to do it, the PCR Rees-George et al. (2010) or Gallelli et al. (2011).I can only get one of these two protocols because they are the 2 indicated by EPPO.
This is where you evaluate what they did, and try to determine what best fits your needs, or fits in your budget/time constraints. You have two sources, and a description of the protocols . . . why do you think a consensus on a "social site" would be better than your own evaluation, or that of your advisor/colleagues where you are located?
Not necessarily helpful, but that is how science operates.
James thanks for your answer. I work in a Laboratory screening not on investigation in a small Island called Azores.My colleagues from Mainland are a problem. That's unfortunately my reality. My country is in crisis. Thanks for your time.Best regards, Manuela
Manuela,
Sorry that things are as they are and that you cannot get any assistance from your colleagues.
If you could give the full citation of the manuscripts, and what specifically are your needs then perhaps it might be easier to assist you.
Hi James, thanks for your time, but I contact the official lab in Galizia (Spain) and they work with this protocols. I get the answer that I need. On more time, thanks for your attention, Best regards, Manuela
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