Hi All,

I have recently done some electroporation into GS115 strain with pPIC9 containing a 2230 bp fragments. After transformation I performed colony PCR with gDNA from Pichia and used plasmid DNA(my template) as positive control. I have attached an image from AGE.The band intensity is quite different from the PCR with plasmid DNA.

If you look at the image you can clearly see the PCR band intensity difference .

Fast one is ladder, 2to6 is transformant, 7 negative control, 8 PCR from plasmid containing the insert.

Do you think the PCR worked?