hello, looking for a paper or study looking at the difference, best method or comparison using GFF filters vs PCFE?
Is the acetone extraction can be done the same way (>48h in acetone wihtout mechanical abrasion?)
thanks
Let me know if you have any experience in this subject or any interest. Also I need some articles about this field and authors who worked in this field.
26 February 2023 9,275 4 View
how to add a new Journal name not listed in the fields for a new article uploaded?
18 January 2021 7,215 3 View
I'm interested in the dissected wet brain volumes and also the total volumes CSF included!
30 January 2019 9,696 0 View
Hello, I am using probes and for calibration I was supposed to use sodium sulfide nanohydrate but my order came wrong and I have only sodium sulfide (Na2S) or sodium sulfide hydrate 60% (H-Na2S)...
19 August 2018 4,848 1 View
I purify antibodies for conjugation with dyes for IF staining on tissues, and use HiTrap protein A and G HP columns for purifying.
25 February 2016 8,172 8 View
Hi, got a data set of three variables set as presence/absence (ie 8 possibilities) among 1200 stations and I would like to run a DISTLM model on Permanova (Primer) but I am not sure if we can run...
02 August 2015 8,275 4 View
Any researcher who is familiar with linguistic anthropology,gesture,signs or any researcher who is into communication department.
16 March 2015 1,829 13 View
I have found only a handful of studies in my review of literature. Just wondering if anyone is looking at this also.
30 January 2015 8,523 4 View
I am looking for a protocol to sample and analyze flocculent matter (anaerobic, gases, etc).
13 October 2014 4,232 1 View
The evaluation of diastolic function is very important carrying prognostic informations. While in adults there are guidelines and statements in children there are less evidence. So in your...
11 July 2014 520 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View