In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation.

I would like to know if, after isolating the cells and before the infection, I should plate the cells in culture medium with fetal bovine serum or without the serum.

I will be plating 1x10^6 cells per well, and I will only infect them the following day.