Design a primer?

Hi, I am trying to design primers for attached sequence with resulting product length of 800-1000bp. But could not design any suitable primers. The tools I used are as...

10 November 2018 7,125 7 View

Are honeybees vector to different plant pathogens?

09 October 2014 9,317 9 View

How can we stain different genomes with immunofluorescence techniques?

Some insects, like rice weevil (Sitophilus oryzae), have four intracellular genomes i.e. nuclear, mitochondrial, principal endosymbiont, and Wolbachia. Can we stain these different genomes with...

10 November 2013 1,986 0 View

How can we differentiate extra cellular proteins produced by some microbes from the protein produced by an organism itself?

There are some microbes especially gurmicrobiota that perform different functions from food digestion to defence against pathogenic bacteria. They also produce some enzymes, antimicrobial peptides...

10 November 2013 7,524 4 View

Can we use immunofluorescence technique for mitochondrial DNA or RNA?

Fluorescence techniques are mostly used to identify the intracellular organs like nucleus, microtubules etc. Can we stain mitochondrial DNA or RNA from these techniques?

10 November 2013 7,814 1 View

Paratransgenesis for improving honeybee colony health?

Can we use microbiota for paratransgenesis in the beekeeping industry to control American foulbrood?

09 October 2013 4,124 0 View

Citation numbers differ on Research gate and Google Scholar profile

Since long I am observing that number of citations to my research publications differ on Research gate and google scholar profile. Is this happening with you also? Can anybody suggest that how can...

01 January 1970 7,279 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View