Can anyone suggest the name or contact details of commercial laboratories offering chemical analysis of organic materials through LC-MS/MS?

I have plant methanol, ethyl acetate, n-hexane, and butanol extracts. I am interested in the chemical profiling of each extract through LC-MS/MS. If someone knows the details of commercial labs...

31 December 2019 1,508 4 View

Design a primer?

Hi, I am trying to design primers for attached sequence with resulting product length of 800-1000bp. But could not design any suitable primers. The tools I used are as...

10 November 2018 7,125 7 View

Are honeybees vector to different plant pathogens?

09 October 2014 9,317 9 View

How can we differentiate extra cellular proteins produced by some microbes from the protein produced by an organism itself?

There are some microbes especially gurmicrobiota that perform different functions from food digestion to defence against pathogenic bacteria. They also produce some enzymes, antimicrobial peptides...

10 November 2013 7,524 4 View

Can we use immunofluorescence technique for mitochondrial DNA or RNA?

Fluorescence techniques are mostly used to identify the intracellular organs like nucleus, microtubules etc. Can we stain mitochondrial DNA or RNA from these techniques?

10 November 2013 7,814 1 View

Paratransgenesis for improving honeybee colony health?

Can we use microbiota for paratransgenesis in the beekeeping industry to control American foulbrood?

09 October 2013 4,124 0 View

Citation numbers differ on Research gate and Google Scholar profile

Since long I am observing that number of citations to my research publications differ on Research gate and google scholar profile. Is this happening with you also? Can anybody suggest that how can...

01 January 1970 7,279 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View