What are the parameters (biochemical and physiological) that can be investigated under plant salinity stress?
Nice question. There are two pdinciple usually adopted to evaluate the response under salinity stress..These include : accumulation of sodium or chloride and yet plants express at optimum and the other strategy entails about the sodium or chloride exclusion behavior , thereby, reducing the oxidative stress to plant. It would be more interesting to see , how plants respond to such salinity induced oxidative stress biochemically .
Proline accumulation is important parameter to reckon with the salinity respinse. Other polyamide dynamics also play a decisive role in salinity response. But still I feel, simulating the natural salinity condition in terms of salt composition is more important. Application of OMICS tools also find good application .
Dear @Girija Prasad Patnaik
Salinity is a common abiotic stress that severely limits crop growth and development, productivity and causes the continuous loss of arable land resulting in desertification in arid and semi-arid regions of the world. Salinity tolerance in many crops including rice is predominantly associated with the maintenance of ion homeostasis, particularly low Na+/K+ or high K+/Na+ ratios, through exclusion, compartmentation, and partitioning of Na+. In addition to ion homeostasis strategies, many plants have evolved mechanisms to regulate the synthesis and accumulation of compatible solutes such as proline and glycine betaine, which function as osmoprotectants and have a crucial role in plant adaptation to osmotic stress through stabilization of the tertiary structure of proteins.
https://www.sciencedirect.com/science/article/pii/S1319562X1500114X
However, physiological and biochemical parameters for salinity tolerance may vary depending on crops. That what is applicable to cereals may not apply to pulses.
https://www.sciencedirect.com/science/article/pii/S2214514117301162
https://www.researchgate.net/publication/236340791_Breeding_for_abiotic_stresses_in_pigeonpea
Well parameters that you can test are as follows: plant growth attributes (Length of the Shoot system, Number of plant leaves, Leaf area), relative water content, photosynthetic pigments, proline, sugar, protein, ionic content of Na+ and Cl- contents.
Parameters that can be recorded under plant salt stress ( Salinity stress) experiments/ research are growth, physiological, biochemical parameters and nutrients:
Article Effects of salinity stress on some growth, physiological, bi...
First of all, you must properly choose saline treatments, including NaCl, KCl, (NaNO3, KNO3 also in my opinion), PEG8000 or similar to induce an osmotic effect at a similar osmotic pressure (MPa) to the other inorganic salt treatments.
I want to recommend this article, you can follow this parameters measurement.
Article Osmolyte Accumulation and Sodium Compartmentation Has a Key ...
Good luck
Hi,
Please follow the link below for your queries. This paper has all the physiological parameters that can be recorded:
Deleted research item The research item mentioned here has been deleted
You can email or message me for detailed M&Ms.
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