Hello Friends,
I look for a paper/book/website to study about the relationship among salt bridges / proteins/genetics and evolution. I have searched alot but I failed.
Can you please advise me?
Best,
Salt bridges are frequently investigated in the context of thermophilic organisms: try this search:
https://scholar.google.ch/scholar?q=thermophilic+proteins+salt+bridges&hl=en&as_sdt=0,5
Hi Maryam
This is a cool paper about molecular evolution of proteins. Check it out... they talk about salt bridges
http://dx.doi.org/10.1016/j.cub.2006.10.063
Be free to continue discussion. Very interesting topic indeed... Probably you should look to classical old papers about protein structure.
Best
Paco
Dear Dr. Francisco,
Thank you very much for your answer. I am gonna start reading the paper you introduced and I will get back here to ask my questions.
Hey Guys, I have a ubiquitin, I am planning to claculate radius of gyration for residue 1 to 24 and 25 to 76. How can I make this? What command shall I use? Thank you. Maryam
11 December 2017 3,480 3 View
Hey Guys, I am calculationg radius of gyration for 1ubq. My reference file is a pdb file. and I cannot change it. So I have to deal with it!! The problem is that I receive 2 warnings: WARNING:...
11 December 2017 5,279 6 View
Hi friends! I am running my simulations in negative ion mode, gromacs. The problem is that, my proteins completely unfold, and rotate badly. At the very begininng they start unfolding and...
10 November 2017 6,089 1 View
Dear friends, I have a problem with gromacs. My protein rotates in md simulations. I run the simulation in 300K but the protein rotates and suddenly starts doing weird things! It completely...
09 October 2017 1,651 7 View
Hello! How can I calculate radius of gyration for the proteins using .gro files. We use .tpr and .xtc files to calculate R of G but right now I need to use my .gro files. Thank you.
07 August 2017 3,153 4 View
Hello friends! I have 100 .gro files. How can I combine them and create just one .gro file? Thank you! Maryam
07 August 2017 8,847 1 View
Hello friends, I am really really desparate right now! I am working in negative mode in Mass spec. I added formic acid+acetonitrile+water to my protein and I didn't receive any signal. I added...
02 March 2017 3,827 10 View
Hello everyone! I have 1 central protein in pymol with 6 surrounding proteins. I want to find a command to say: please select nterm only on central protein. How can I do this? Thank you.
01 February 2017 1,081 0 View
Hi! How can I select only 1 atom in pymol? only 1 atom, instead of selecting residues. Thanks
01 February 2017 5,771 1 View
Suppose that I have measured distances between positive and negative sides and all of the proteins are in cartoon format. Now, I want to hide the whole proteins except the charged chains. I mean,...
01 February 2017 9,241 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View