WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Do you have experience visualising multiplex amplification curves in LightCycler 480 Sw??

We are using Cobas z480 for multiplex RT-PCR using 3 taqman probes FAM/VIX/CY5. Once the PCR finish we want to see in the amplification curve the three results for each sample at the same time....

28 October 2020 8,372 1 View

TEACHING ESL in the lockdown - participants needed?

Dear English lecturers (teachers, instructors), I am conducting research into ESL teaching to increase our understanding of how teachers have experienced special teaching conditions during the...

14 October 2020 7,223 6 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

11 August 2020 8,076 3 View

What psychometric properties should be reported for a translated version of a questionnaire?

Dear collegues, I performed a cross-cultural study using two questionnaires (66 and 12 items). The original version of these questionnaires was used in the first country, and these tools were...

27 April 2020 4,097 9 View

USRP N210_ RF1 and RF2 dont work ?

Hi, I am working on USRP N210 and Matlab 2016R. I want a transfer a Sinusoidal wave from one port of Antenna and want to receive same wave from second Antenna at one USRP N210 (unfortinaly I...

28 January 2020 5,568 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

How to select substrate concentration to determine the inhibitor behavior as competitive or non-competitive?

I already calculated the IC50 value of the inhibitor against the protease enzyme. Now I need to find this inhibitor competitive non-competitive. I decided to perform the Lineweaver Burk plot, but...

21 January 2021 8,378 3 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

How to dissociate and culture retinal cells from mouse eyes?

We have a transgenic mouse line in the laboratory from which we are looking to culture retinal cells from. Our experiments indicate we might be able to produce stable cultures from these cells...

03 January 2021 7,200 6 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

What are some factors that would result in a small fEPSP?

Hi everyone, I'm consistently having small fEPSP's of approximately -0.01 mv. I've been moving the position of my electrodes and increasing the stimulation intensity but the response doesn't...

03 December 2020 7,125 7 View

What is an appropriate tip diameter size for a stimulating electrode?

I'm setting up an electrophysiology rig examining LTP in the CA1 region of the hippocampus. What is an appropriate tip diameter size for a stimulating electrode to generate fEPSP? Also, I'm...

01 December 2020 5,139 2 View

Can TEV protease cleave between glutamine and methionine efficiently?

Based on this paper The P1 ' specificity of tobacco etch virus protease ,TEV protease can cleave between the Gln and several amino-acids (besides Gly/Ser) with acceptable efficiency in its...

26 November 2020 5,987 4 View