Pacbio sequences and DADA2 pipeline compatible?

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After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

Is there any software/code for studying the effects of wind on vegetation?

I'm searching a (free) software that simulates the effect of strong wind on the vulnerability a tree.

15 December 2020 8,119 3 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Do you know a reference on action of Heisenberg-Weyl algebra on some basis states?

I am looking for references on Heisenberg-Weyl algebras, namely I am interested in the action of algebra elements on some basis states, i.e. matrix elements in a certain basis.

12 November 2020 181 3 View

Should I share my Doctoral dissertation with a Creative Common License?

Disseminating your PhD dissertation. I am working on publishing activity from my PhD thesis, for which I have carried out three different qualitative investigations. I am extrapolating case...

30 September 2020 7,119 4 View

KCL, NaCl crystalline, NH4Cl, PEG in MOPS with BSA and Procline 300?

Why do we need all three salts in MOPS buffer? and PEG added to the reagent. NaCl crystalline 1.359 g/L KCl 0.708 g/L Ammonium chloride 0.0063 g/L KCL...

26 April 2020 6,080 4 View

Does anyone use/have/try any antibody anti OVGP1 in oviduct cells/tissue?

I am looking for an antibody to show the expression of OVGP1 in porcine oviduct cells in culture. Is there one from Sigma and others form ABCAM. But an antibody is too expensive (at least for us,...

31 March 2020 7,273 2 View

Primer Specificity SARS-COV, 2019nCOV?

I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two...

30 March 2020 9,084 3 View

Competitive immunoassay prinicple?

A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....

16 March 2020 9,894 3 View

Will e-beam deposited cobalt etch in HCl?

Hi, I have a sample of e-beam deposited cobalt that I dipped in 1:1 HCl:H2O at room temperature. The cobalt did not etch. Cobalt is supposedly easily etched with most acids, so I am confused why...

12 March 2020 6,301 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Abhijeet Singh

https://benjjneb.github.io/LRASManuscript/LRASms_fecal.html

https://benjjneb.github.io/LRASManuscript/LRASms_HMP.html

https://benjjneb.github.io/LRASManuscript/LRASms_Zymo.html

Lorenzo Pin

Thank you very much!

Muhammad Umar Sohail

Please refer to this GitHub tutorial.

I hope it will help