I am looking for few p53 wild type tumor cells that express high and/or low levels of Mdm2. In the literature I found that liposarcoma cells expresses high levels of Mdm2, but these cells are not available commercially. Does someone know about such cells which are also available commercially. Thanks in advance for all your help.
Hi Sajid,
Previously I have used HCT116 colon adenocarcinoma which are TP53 wild-type and express MDM2 nicely. For more cell types you can look at the morpheus cell line encyclopedia database https://software.broadinstitute.org/morpheus/
Best wishes,
Teresa
Hi Sajid,
you could by NT2 TGCT cell line at ATCC.
https://www.ncbi.nlm.nih.gov/pubmed/?term=19560254
You could also Ask J. Fletcher's lab in Harvard if they could provide LPS141.
Otherwise get back to me by EMail. We got also the cell lines you need.
Best regards,
Thomas
Hi Sajid, you can use two human neuroblastoma cell lines: NGP and NB19, which expresses high and low Mdm2.Both of the two cell lines are p53 wild type. You can check this article:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920959/
Thank you Drs. Roposo, Muhlenberg and Chen for your answers.
Dr Muhlenberg- Thank you so much for your generosity. I will try to contact Dr Fletcher's lab if they could share LPS141 with us. Alternatively, would it be possible for you to ship the the cells to my place?
Dr Chen- Do you know if these cells commercially available? I searched on the ATCC website and could not find these cells.
Thanks
Sajid