Hi, is it okay to use a construct expressing a rat gene, instead of the required mouse gene if both of them produce 100% homologous protein. Has anybody done that?
Cas9 is from bacteria, GFP is from Jellyfish. Technically it is OK. As far as the gene is functional and can be expressed in the cell, you can use the rat gene in mouse straight away. Lots of people transfected human gene into mouse primary neurons.