Vineeth A Raveendran

4 Questions 6 Answers 0 Followers

Questions related from Vineeth A Raveendran

Using pCMV-eGFP as an empty control for pCMV-ProteinX-GFP?

I have a really old construct that contains GFP (original) tagged protein under CMV promoter. I can't find an empty vector containing GFP (Addgene or similar). The closest they have is eGFP under...

03 March 2019 9,638 4 View

Overexpressing Rat gene in mouse primary neuronal cultures?

Hi, is it okay to use a construct expressing a rat gene, instead of the required mouse gene if both of them produce 100% homologous protein. Has anybody done that?

01 January 2019 1,125 1 View

Quantification of mitochondrial elongation in Image J ?

Hello,I am trying to quantify the length of elongated mitochondria using ImageJ. I have been using Ferret diameter, but I guess this gives me an underestimation of actual length. Is there any way...

07 July 2016 6,796 2 View

How to generate topology file of an arsenic compound for gromacs simulation?

I have tried many online tools, but most are for light elements. Thanks in advance.

01 January 2016 1,552 3 View