Hi

I am doing amplicon based sequencing by mini seq of about 18 targeted genes of mycobacterium tuberculosis. But each time i faced a problem of overcluster density. As the optimum cluster density is 200 to 220 but my run cluster density was more than that. I tried to run the samples by changing the concentration of library pool from 1.4 to 1.3. And in my last run i kept that on 1.2 . But there is no change of the cluster density. Moreover it is increasing in 1.2 picomole than 1.3 and 1.4. I am not sure about the reason. So how can i solve that problem and bring my cluster density into optimum range??