Is there Researchers who are already working on anti-viral surfaces. For ex. anti-viral COVID-19 surfaces ? ?

It would be interesting to know, how the scientific community is contributing to the COVID-19 crisis. How chemical/mechanical/material/metallurgy concepts are being used to produce anti-COVID-19...

16 May 2020 5,141 3 View

How to calculate ECC using HFSS software?

Good morning. Now i am designing MIMO antenna for Ultraband Applications. Can anyone suggest how to plot ECC (Envelope correlation Coefficient) Using HFSS 15 software. Thanks in advance

13 February 2020 5,261 6 View

Data structure and missing values in random forest model?

Hi there, I have over 1 million records, with a categorical response variable and a mix of categorical and continuous predictors. There are several missing values in the dataset, this is...

02 September 2019 494 4 View

Tools/techniques to describe the distribution of cells in a raster, across a study region?

Im trying to analyze and extract patterns from several raster layers, something like what is done for non-raster features. Im interested in learning techniques that describe the distribution of...

02 July 2019 2,333 5 View

Can anyone suggest me how to plot fractional order time delay system in matlab?

I am searching a matlab code for fractional time delay system(continuous case). Still now, i don't got any idea for the above. I need a good suggestion from researcher. Help anyone!!!

06 March 2019 6,097 3 View

Selecting a DC Voltage for EIS Measurements: Can someone help me with this ?

Am confused in deciding the DC Voltage to select for EIS measurement of my ss316L (Coated and Surface modified). Just wanted to crosscheck before proceeding forward. I found this article bit...

12 December 2017 4,913 4 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

25 November 2017 8,132 2 View

How to calculate the crossliking density of the rubber?

How to calculate crosslinking density of the rubber and Molecular weight between crosslinks in NR, SBR, etc..

27 June 2017 5,642 5 View

Why the RNA got degraded during purification ?

29 March 2017 1,127 2 View

Is it possible to amplify a single copy of dna template (practically) efficiently by using PCR ?

14 March 2017 3,999 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View