Do anyone has specific primers for genotyping OT2 .Jax primers are not working .I understand FAcS using Valpha 2 will help to screen
I need specific primers for screening
Hi guys, I have been using PyMol as a molecular viewer tool for my docking. There used be an option to isolate a monomer sequence from a dimer/trimer/tetramer complex protein structure. Can anyone...
14 April 2024 2,773 1 View
We have two compounds, which are highly soluble in water even we can't separate through workup or filtration (one is amino acid derived amide and other is amino acid)
21 February 2024 1,678 2 View
Digital Electronics
22 October 2023 7,669 1 View
Computational chemistry
19 October 2023 5,964 4 View
19 October 2023 8,297 1 View
Computational physics and chemistry
12 October 2023 2,303 1 View
from computational physics point of view
07 October 2023 2,771 2 View
the pixels of an image is scanned from left to right and from the top to bottom. each new pixel is predicted by the avg of the pixel above and one to the left.
01 October 2023 4,525 1 View
this is related to DFT
16 September 2023 8,074 4 View
I have only ledit file how to make hard mask for mask aligner. is this possible to do using laser writer or engraver. how to make pattern?
09 September 2023 903 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View