I am looking for an open bioinformatic program or script with the option of quick analyzing the presence or absence of nonsynonymous SNPs in the NGS results of amplicons of known proteins in a particular human individuals. The program of course may include mapping to the reference genomes but in principle it should detect known SNPs by analyzing the nucleotide sequence and finding a substitution in a codon causing a change in a known SNP position (triggering mutation to another amino acid). Mainly known substitutions are important, however detection of novel would be optional with mapping. I possibly would appreciate pointing to a few programs to fix them with a script. And it's not about dbSNP on NCBI or any other.