Hi fellas,
I have used an antibody for detecting a protein sized 37kD. I had a positive control showing it whereabouts 34-35 kD (mouse cell line) and the transfected cell line ( protein overexpressed) showing at 40 kD (bovine cell line). the protein in both species is 373 aa. I was wondering what possibilities could have caused such discrepancy?
Cheers
Vahid