Once a mutation is detected in an individual, how to determine if it is homozygous or heterozygous?

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Juan Pablo Allocati

Muchas gracias Gonzalo!

Sarfraz Kiani

Is it in human?? Otherwise, in plants backcross would help.

Anjali Chayal

try Mutation Surveyor software ..

Vishwanath Koti

We can do it by molecular markers like SSR/SNP.

Ellina Mansyah

Hello...To distinguish homozygous and heterozygous individuals can be performed using molecular analysis. Currently there are many PCR-based molecular markers that can be divided into dominant and codominant markers. Dominant markers (eg RAPD and ISSR) are unable to distinguish between homozygous and heterozygous individuals while codominan markers (eg, RFLP and SSR) able to discriminate betwen homozygous and heterozygous individuals. Therefore, in accordance with the purpose of your research is recommended to use the codominant markers.

María Patricia Benavides

How can I distinguish between homozygous and heterozygous lines in T-DNA mutant Arabidopsis plants?