I’m culturing NIH3T3 cells with DMEM high Glucose. I’ve observed that the media’s pH drops down significantly (yellowing of medium) in less than 24h, subjecting the cells to stress. Thus the cells need media change everyday and also it takes more than a week for it to become 90% confluent.

What can I do to prevent acidification or any suggestions where I might be going wrong and should reconsider those steps.