Incubation time, temperature (-80, -20, 4 ° C, 30 ° C) 500mM EDTA, 6M Urea, Lysozyme (g / ml)?
Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3?
How to upload pdf of my articles published?
03 March 2021 2,183 3 View
03 February 2021 3,756 4 View
Dear Research Gate members, for my masters's thesis, I have conducted a questionnaire to investigate consumer's attitude towards sport sponsorship. I've asked three identical questions...
27 January 2021 2,910 3 View
I am working on solving an optimization problem with two objectives by using neuroevolution. I use NEAT to evolve solutions which need to satisfy objective A and objective B. I tried different...
27 January 2021 4,875 3 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
05 January 2021 1,196 7 View
Graphs like the one attached below can be found on every website explaining the spectral properties of the atmosphere. However, I wasn't able to find the data behind these graphs. I need the...
07 December 2020 2,851 4 View
I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...
30 October 2020 9,077 4 View
What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...
27 October 2020 7,532 5 View
I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match
10 August 2020 699 3 View
In MATLAB, you can use the Vehicle Network Toolbox, which supports CAN interfaces. I'm searching for something similar in Octave.
06 August 2020 9,571 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View