Incubation time, temperature (-80, -20, 4 ° C, 30 ° C) 500mM EDTA, 6M Urea, Lysozyme (g / ml)?
Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3?
I am studying large-scale granite structures, specifically inselbergs. I would like to know your suggestions for analyzing these granite structures using remote sensing. Books, articles, and...
17 June 2024 6,687 1 View
We want to block and potentiate CB1 receptor in tissue through agonists and antagonist "ex vivo". But how we can check that the drug used is working? We can check by WB the levels of the total...
22 April 2024 8,060 0 View
Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...
27 March 2024 7,900 0 View
Me encuentro realizando una investigación sobre La Historia del Comercio Internacional de Colombia, En el Primer capítulo me encuentro abordando los imperios Latinoamericanos precoloniales como el...
21 March 2024 4,148 2 View
I am looking for scales to measure the perceptions of legal risk when developing certain software or information system features.
14 March 2024 7,854 2 View
As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?
03 March 2024 3,653 3 View
HemInozoin cristals are very compact in human parasites. However in opther haemoparasites as order haemosporidia is observed volutin. Do you think is there any correlation between protein...
26 February 2024 3,806 2 View
Hello. I am trying to culture Agrobacterium GV 3103 and EHA105 in 15 mL LB media with the antibiotics kanamycin and rifampicin (100 ug/mL & 20 ug/mL)(added 200 uL of previously normally...
25 January 2024 812 5 View
While performing STD screening I noticed that I have signals in my difference spectras, that are constant accross all samples, also if there is no ligand and only protein. The protein was...
18 January 2024 6,932 2 View
I cannot see the difference between these two types of resins that contain iminodiacetic acid as functional groups besides that the IMAC resins contain the metal for the His-tag. Is this the only...
14 January 2024 8,378 5 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View