Sebastian Hurtado Cortes

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Questions related from Sebastian Hurtado Cortes

Plasmodium falciparum evades immunity of anopheline mosquitoes by interacting with a Pfs47 midgut receptor?

If you have read this article I need your help please. What is a protein with natterin structure? What characteristics does it have? Why is a UI control used, Lacz? Does the mosquito produce...

20 May 2020 1,566 0 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

25 April 2020 4,445 3 View

Can 6M Urea inhibit lysozyme action? or this happens the other way around?

I mix both components together with other substances to treat my bacterial pellet and extract protein, but I don't know if Urea 6 M or lysozyme loses its effect

25 April 2020 4,246 14 View

Of these components which can degrade the recombinant protein? what degrade my recombinant proteins?

Incubation time, temperature (-80, -20, 4 ° C, 30 ° C) 500mM EDTA, 6M Urea, Lysozyme (g / ml)? Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3?

25 April 2020 4,313 0 View

Can the Urea 6M buffer slow down the action of lysozyme? How can I stop the lysozyme reaction? Can lysozyme degrade my recombinant protein?

After centrifuging my bacteria, I added to the E. coli BL21 DE3 pellet: EDTA 1mM, Urea 6M, lysozyme (1mg / ml), PMSF 1mM, and commercial protease inhibitor tablets 1X diluted in water. At 4 hours...

04 April 2020 9,373 0 View