oes anybody have experience to share about Biotek microplate readers?
I have a good amount of experience with the Biotek Synergy H4 plate reader and Gen5 software. What do you want to know?
Hi!, I have in my lab two Biotek microplate readers, the Sinergy H1 and the ELx808. Let me know what you want.
yes i have exp with biotech reader and also gen5 software.pretty happy with it.
what you want to know exactly
My experience is quite satisfactory with a Sinergy HT and Gen5 software. More than 5 years without technical problems, and good performance.
Thanks everybody!
That was quick. I have used SpectraMax from Molecular Devices for 20 years and I am about to buy a Biotek Synergy H1MF and my associate Russell and I wanted to be sure this is a good, reliabe piece of equipment and the software is user-friendly.
Thanks in advance
Alejandro
Hi Alejandro,
I have now used plate readers from 3 different companies. Gen5 software is by far my favorite and is, in my opinion, the most user-friendly of them all (Biotek, Molecular Devices, Promega). The instant-output to Excel is fantastic.
Looking at the spec sheet it looks like the H1MF is the current model equivalent of the Synergy H4 that I used previously. This was a an excellent instrument that was highly flexible for the various assays that I ran. I used the instrument for fluorescence, fluorescence timecourse, absorbance, absorbance timecourse and luminescence very frequently. My favorite features were (1) the monochrometer, allowing you to dial in the exact wavelength you want for absorbance and fluorescence, with the available filters for standard wavelengths and (2) the spectral scanning ability. The filters will be more sensitive, but the flexibility of the monochrometers is unbeatable. The spectral scanning was helpful validating FRET experiments as well as characterizing some of the new chemicals we were developing.
Hey guys I have a GEN 5 question. I measure absorption between 400 and 700 nm. Is there a quick way to get an excel output for each sample at each wave length? Copying every single nm in 1 nm steps is a torture. I hope my question is clear. Thanks in advance. Cheers
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