Hi
i am working on the induction of focal cerebral ischemia in rats of different weight. I have tried various un-coated sutures (for my pilot scale study) like polypropylene 3-0, polypropylene 2-0, nylone 4-0 but the best result so far is provided by polypropylene 3-0. i don't know why is a nylone 4-0 so widely accepted as the standard for induction of cerebral ischemia. can anybody explain the factors the govern the choice of the suture?
It generally has to do with the caliber of the MCA in rats, the 4-0 produces a reliably consistent occlusion.
It has been my experience (see Minematsu et al, Neurology 42:235-240, 1992) that 4-0 nylon, with the proposed distal tip rounded by near-flame heating, introduced via the CCA reliably occludes the ipsilateral MCA due to size match and produces a consistent infarct size in Sprague-Dawley rats weighing 280-350 grams.
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